Journal: Genome Biology

Article Title: Dynamic inosinome profiles reveal novel patient stratification and gender-specific differences in glioblastoma

doi: 10.1186/s13059-019-1647-x

Figure Lengend Snippet: Clinical relevance of editing at COG3 I/V recoding site. a Kaplan-Meier curves representing the survival probability of GBM patients stratified by COG3 I/V (104 samples) and CADPS E/G (108 samples) editing levels respectively. Log-rank test, COG3 p value = 0.044, CADPS p value = 0.56. The red line represents high editing frequency (≥ 40%), the green line represents low editing frequency (< 40%). b Migration assay of glioblastoma cells (A172) expressing unedited COG3 (uned) and edited (ed) COG3 was performed 24 h post-seeding. Representative photographs of migrated cells are shown (× 4 and × 10 magnifications). Migrated cells were stained with Diff-Quick and counted. Histograms show the migration ability of ed. COG3 expressing cells relative to the uned COG3 (fold increase ± SD, n = 3) ** p ≤ 0.01 (two-sided t test). c Proliferation (MTS assay) of glioblastoma cells (A172) infected with unedited or edited COG3 (mean ± SD, n = 3) ** p ≤ 0.01 (two-sided t test)

Article Snippet: Human GBM cell lines U87-MG, U118-MG, and A172 were obtained from the American Type Culture Collection (ATCC) and routinely maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (Gibco-Life Technologies), 100 U/ml penicillin, and 100 μg/ml streptomycin, at 37 °C in 5% CO 2 .

Techniques: Migration, Expressing, Staining, Diff-Quik, MTS Assay, Infection

Journal: Theranostics

Article Title: Blockade of phosphotyrosine pathways suggesting SH2 superbinder as a novel therapy for pulmonary fibrosis

doi: 10.7150/thno.72269

Figure Lengend Snippet: SH2 superbinder exhibited excellent security by targeting lung fibroblasts based on pY levels in vivo. A , Schematic showing progression of SH2 superbinder treatment in control mice. B , Percent survival during last 7 days after SH2 superbinder injection (n = 8). C , HE staining after SH2 superbinder challenge. Images showing the panoramic and partial view of lungs. D , Neutrophil accumulation measured by MPO assay in lung tissues after GST, GST-SH2 WT, GST-SH2 TrM or SH2-TrM treatment in saline groups (n = 6). E , Total cell counting in BALF (n = 6). F-G , Changes in different kinds of cells by Giemsa and Diff-quick staining in BALF (n = 6). H , Inflammatory cytokines in BALF measured by ELISA (n = 6). I , The determination of liver function index in serum (n = 6). J , Schematic showing progression of SH2 superbinder treatment in BLM-treated mice. K , Western blot of GST tag of different tissues in BLM group treated with GST-SH2 TrM. L , The process of FACS for mice lungs. M , Western blot of pY levels in different lung cells which were isolated by FACS of BLM treated 14 days mice. N , The fluorescence images of SH2 superbinder entering into activated fibroblasts, epithelial cells (EpCAM + ), leukocytes (CD45 + ) and endothelial cells (CD31 + ). O-R , Representative images of SH2 superbinder in myofibroblast (α-SMA + ) ( O ), epithelial cells (EpCAM + ) ( P ), leukocytes (CD45 + ) ( Q ) and endothelial cells (CD31 + ) ( R ) of BLM-treated mice.

Article Snippet: The amounts of IL-1β, IL-6, IL-10 and TNF-α in BALF of mice were measured by using mouse ELISA kits from R&D Systems (Minneapolis, MN, USA).

Techniques: In Vivo, Injection, Staining, MPO Assay, Saline, Cell Counting, Diff-Quik, Enzyme-linked Immunosorbent Assay, Western Blot, Isolation, Fluorescence